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The present study was undertaken to investigate the effects of Agaricus blazei murill polysaccharides on morphology of hippocampus tissue and expression of ASIC1a and ASIC2b mRNA in lead-poisoning rats.Forty SD rats were divided into five groups:control group,lead group,experimental group 1,2,3.After 60d of treatments,hippocampus tissues were observed by HE staining and the expression levels of ASIC1a and ASIC2b mRNA were determined by the flouoreescent quantitative real-time polymerase chain reaction(FQRT-PCR).The results showed that after lead induction,the number of hippocampus neuron increased,cell gap increased,the chromatin was stained lightly;after administration of ABMP,the number of the hippocampus neuron increased as the dose of ABMP rose,cell gap decreased,the chromatin was stained darkly.The expression level of ASIC1a mRNA in hippocampus of rats in ABMP group decreased extremely significantly compared with that in the model group(P<0.01).Lead exposure and ABMP exhibited no significant effects on the expression level of ASIC2b mRNA.ABMP possesses a protective effect on nervous system of rats exposed to lead by combining with lead.
The present study was undertaken to investigate the effects of Agaricus blazei murill polysaccharides on morphology of hippocampus tissue and expression of ASIC 1a and ASIC2b mRNA in lead-poisoning rats. Forty SD rats were divided into five groups: control group, lead group, experimental group 1 , 2, 3. After 60 days of treatments, hippocampus tissues were observed by HE staining and the expression levels of ASIC1a and ASIC2b mRNA were determined by the flouoreescent quantitative real-time polymerase chain reaction (FQRT-PCR). The results showed that after lead induction of the number of hippocampus neuron increased, cell gap increased, the chromatin was stained lightly; after administration of ABMP, the number of the hippocampus neuron increased as the dose of ABMP rose, cell gap decreased, the chromatin was stained darkly. expression level of ASIC1a mRNA in hippocampus of rats in ABMP group decreased extremely significantly compared with that in the model group (P <0.01). Lead exposure and ABMP exhibiting no significant effects on the expression level of ASIC2b mRNA. ABMP possesses a protective effect on nervous system of rats exposed to lead by combining with lead.