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目的 观察男性不育病人精浆弓形虫IgG与NO水平及其关系。 方法 采用ELISA方法测定 16 9例男性不育患者及 35例正常生育男性精浆弓形虫IgG抗体 (ATAb)和抗精子抗体 (AsAb) ,同时采用硝酸还原酶法测定精浆中NO水平。结果 男性不育病人精浆ATAb阳性率 18 35 % ,显著高于正常生育组的 2 86 % (P <0 0 5 ) ;男性不育组精浆AsAb阳性率为2 6 6 3% ,也显著高于正常生育组 (P <0 0 1)。男性不育组精浆中NO水平 14 6 6 8± 38 87μmol/L ,显著高于正常生育组 84 92± 2 6 72 μmol/L(P <0 0 1)。精浆弓形虫IgG抗体阳性的男性不育病人精浆NO水平 15 3 2 1± 4 1 6 0 μmol/L ,显著高于精浆ATAb阴性组的男性不育病人精浆的NO水平 135 81± 31 4 5 μmol/L(P <0 0 1)。精浆AsAb阳性的男性不育病人NO水平15 8 76± 4 3 0 0 μmol/L ,显著高于精浆AsAb阴性组的男性不育病人精浆的 133 82± 32 10 μmol/L(P <0 0 1)。精浆ATAb阳性的男性不育病人精浆AsAb的阳性率为 38 71% ,也显著高于精浆ATAb阴性组的男性不育病人精浆AsAb的阳性率2 3 91% (P <0 0 1)。结论 男性不育病人精浆ATAb与精浆NO水平具有相关性 ,同时也与抗精子抗体的产生可能有着一定的关系。
Objective To observe the levels of IgG and NO in seminal plasma of male infertility patients and their relationship. Methods ELISA method was used to determine the serum levels of ATAb and anti-sperm antibody (ASAb) in seminal plasma of 169 male infertility patients and 35 normal fertile men. Nitric acid reductase was used to determine NO level in seminal plasma. Results The positive rate of seminal plasma ATAb in male infertility patients was 18 35%, which was significantly higher than that in normal fertility groups (P <0.05). The positive rate of AsAb in male infertility group was also 266.3% Higher than the normal fertility group (P <0.01). The level of NO in seminal plasma of male infertility group was 14 6 6 8 ± 38 87μmol / L, which was significantly higher than 84 92 ± 2 6 72 μmol / L (P 0 01) in normal fertility group. Seminal plasma NO levels in seminal plasma of Toxoplasma gondii IgG positive patients were 15 3 2 1 ± 4 1 6 0 μmol / L, which were significantly higher than those in male infertility patients with seminal plasma ATAb negative 135 135 ± 31 4 5 μmol / L (P <0.01). The NO level in seminal plasma of AsAb-positive male patients was 15 8 76 ± 4300 μmol / L, which was significantly higher than that of the seminal plasma of 133 82 ± 32 10 μmol / L male infertility patients with seminal plasma AsAb negative (P < 0 0 1). The positive rate of serum AsAb in seminal plasma ATAb-positive male infertility patients was 38 71%, which was also significantly higher than that of seminal plasma AsAbs in male infertility patients with seminal plasma ATAb negative group (23.9%, P <0.01 ). Conclusion There is a correlation between ATR in seminal plasma and NO in seminal plasma in male infertility patients, and may also be related to the production of anti-sperm antibodies.