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目的:分析河南栽培罗勒(Ocimum basilicum L.)和丁香罗勒(Ocimum gratissimum L.)成龄叶香气成分的差异。方法:于2015年7月在云南师范大学呈贡校区随机采取长势健壮、无病虫害的河南栽培罗勒和丁香罗勒的成龄叶(自下而上第5、6片叶),采用气相色谱-质谱联用(GC-MS)进行检测;根据峰面积归一化法测定各个组分的相对百分含量。结果:河南栽培罗勒中检出24种香气物质,丁香罗勒中有26种。其中,1,3-二甲氧基苯、石竹烯、乙苯等是2种罗勒共有的主要香气成分。香叶醛、β-蒎烯、薄荷二烯是河南罗勒的主要特有香味成分;蒿脑、桉树脑及异龙脑是丁香罗勒的特有成分。结论:河南栽培罗勒和丁香罗勒种的叶片中香气成分的种类和含量存在明显差异。
OBJECTIVE: To analyze the differences of aroma components in aged leaves of Ocimum basilicum L. and Ocimum gratissimum L. Methods: In July 2015, Chenggong campus of Yunnan Normal University was randomly selected to grow healthy, pest-free Henan-growing basil and clove basil leaves (bottom-up 5,6 leaves), using gas chromatography-mass spectrometry Combined with GC-MS detection, the relative percentage of each component was determined by peak area normalization. Results: 24 kinds of aroma substances were detected in the basil of Henan and 26 kinds of clove basil. Among them, 1,3-dimethoxybenzene, caryophyllene, ethylbenzene and other two types of basil are the main aroma components. Geraniol, β-pinene and menthane are the main fragrant components of basil in Henan. Artemisia, eucalyptol and isoborneol are the specific components of clove basil. Conclusion: There are significant differences in the kinds and contents of aroma components in the leaves of cultivated basil and clove basil cultivars in Henan.