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现存很多高尔基染色法有成功率低、产生沉淀或操作程序复杂等缺点。本文报道了我们发展的显示大脑皮层神经元树突棘的另一种Gogli-Cox染色方法。其方法是将动物灌注固定后取脑并将全脑浸于Golgi-Cox液中2周,然后浸于30%蔗糖中;反应采用100微米厚的震动切片,经过蒸馏水洗涤、氨化、酸性坚膜定影液反应、蒸馏水再洗涤,然后切片经上升梯度酒精脱水、透明,裱片后观察。结果显示,神经元的形状和树突棘标记十分清楚;相比之下,我们采用的另一种应用K2S的Golgi-Cox法则标记质量很差。本研究结果提示我们使用酸性坚膜定影液的变通Golgi-Cox染色法是一种简单而有效的标记神经元树突棘的方法。
There are many disadvantages of the existing Golgi staining methods such as low success rate, precipitation, or complex procedures. Here we report another Gogli-Cox staining method that we have developed to show dendritic spines of cerebral cortical neurons. The method is that the animals are perfused and fixed, the brain is taken out and the whole brain is immersed in Golgi-Cox solution for 2 weeks and then immersed in 30% sucrose. The reaction is carried out by shaking slices with a thickness of 100 μm, washing with distilled water, ammonifying, Membrane fixer reaction, distilled water and then washed, and then sliced by the gradient of alcohol dehydration, transparent, observed after mounting. The results showed that neuronal shapes and dendritic spine markers were clearly defined; in contrast, the Golgi-Cox law of K2S, which we applied, was of poor quality. Our results suggest that our alternative Golgi-Cox staining method using acidic fixer solution is a simple and effective method to label neuronal dendritic spines.