论文部分内容阅读
为了调查流行性出血热(简称EHF)在人群中的隐性感染,我们于1982年7月至1985年6月对全省43个市县(占全省50％以上)进行了健康人群血清中EHF特异性抗体IgG的检测,共检血清5341份,其中发现阳性193份。现将结果报告如下。一、材料与方法 1.标本来源:43个市县健康人群中,经无菌操作静脉采血2ml,分离血清待检。 2.细胞抗原片:由安徽省医学科学研究所提供:A_1株、A_(96)株、陈株感染的A-_(549)及Vero～E_6细胞制备的抗原片。 3.荧光抗体:羊抗人IgG、IgM系上海生物制品研究所生产。 4.方法:间接免疫荧光抗体染色法。 5.判断标准:EHF·V·IgG抗体测定血清
In order to investigate the latent infection of epidemic hemorrhagic fever (EHF) in the population, we conducted a survey in 43 cities and counties (over 50% of the province) from July 1982 to June 1985 in the serum of healthy people EHF-specific antibody IgG detection, a total of 5341 sera were seized, of which 193 were found positive. The results reported below. First, the material and methods 1 specimen source: 43 cities and counties in the health population, by aseptic venous blood 2ml, serum was seized. 2. Antigen Cellular Antigen: Antigen sheet prepared by Anhui Institute of Medical Sciences: A_1 strain, A_ (96) strain, Chen A-infected A-549 and Vero-E_6 cells. 3. Fluorescent antibody: goat anti-human IgG, IgM Shanghai Institute of Biological Products produced. 4. Methods: Indirect immunofluorescence antibody staining. 5. Criteria: EHF · V · IgG antibodies were measured serum