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用于组织学、组织化学和免疫组织化学等方面的制片技术,常需对组织进行固定。固定的目的是尽可能使组织保持原有的形态结构和化学成分。固定的方法有多种,较常用的是灌注固定,即通过心室灌注让固定液沿血液循环的通路快速充满组织之间,使组织细胞的固定均匀一致。灌注固定需用的装置,市场上很难买到。我们根据通常使用的灌注装置原理自制了一套灌注固定装置,经过实际应用,效果良好。现将具体制作方法简介于下(见附图所示): 1.取2500m/细口瓶一个,锯掉瓶口和瓶底,使瓶身高度为17cm,作为套瓶。 2.取500ml塑料输液瓶2只作吊瓶,其
Histology, histochemistry and immunohistochemistry and other aspects of the production technology, often need to be fixed on the organization. The purpose of fixation is to keep the original morphological structure and chemical composition as much as possible. Fixed a variety of methods, the more commonly used perfusion fixed, that is, through the ventricle perfusion fixed fluid along the path of blood circulation quickly filled the tissue between the tissue cells fixed uniform. Perfusion fixed device needed, the market is difficult to buy. We have made a set of perfusion fixing device according to the commonly used perfusion device principle, which has been proved effective by practical application. Now the specific method of making a brief introduction in the next (see the figure below): 1. take 2500m / bottle a, saw the bottle and bottle bottom, the bottle height of 17cm, as a set of bottles. Take 500ml plastic infusion bottle 2 only for hanging bottles, its