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本研究观察了rhIL 11对γ线照射后猕猴血小板和白细胞减少症的治疗作用 ,同时比较了不同时间给药对疗效的影响。用6 0 Coγ射线 3.0Gy照射正常猕猴 ,造成骨髓抑制模型。照射后当天开始给予rhIL 1130 ,60或 12 0 μg·kg-1·day-1,连续 14天皮下注射 ,可以显著提高给药猴外周血小板数的最低值 ,虽然白细胞数最低值并未上升 ,但低于照前值 5 0 %的时间明显缩短 ,这与血小板的变化相似。照射后当天开始给予rhIL 11治疗的动物在照射后开始的 2周内红细胞数较对照组略有下降 ,但第 3周即迅速上升并超过对照组。照射后第 13天开始给予rhIL 11治疗的 4只猴 ,在照射后前 3周内外周血象与对照组相似 ,此后迅速恢复。骨髓细胞培养结果表明 ,rhIL 11治疗猴的骨髓细胞在体外形成CFU Meg ,CFU Mix ,CFU E ,BFU E和CFU GM能力明显增加。照射后第 4 5天病理组织学检查结果表明 ,照射对照组动物骨髓腔空虚并有明显出血 ,治疗组动物骨髓腔中细胞丰富 ,生长旺盛。这些结果表明 ,rhIL 11可以促进照射猕猴造血的恢复
In this study, we observed the therapeutic effect of rhIL-11 on macaque platelets and leucopenia after γ-ray irradiation, and compared the effects of different doses of rhIL 11 on the curative effect. Normal macaques were irradiated with 3.0Gy of 60 Co gamma rays, resulting in a myelosuppressive model. Administration of rhIL 1130, 60 or 120 μg · kg-1 · day-1 on the day after irradiation subcutaneously for 14 consecutive days significantly increased the lowest peripheral blood platelet count in peripheral blood mononuclear cells. Although the lowest white blood cell count did not increase, However, time below 50% of the previous value was significantly shorter, similar to platelet changes. The number of erythrocytes in rhIL 11-treated animals decreased slightly from the control group within 2 weeks after the start of irradiation, but quickly rose beyond the control group in the third week. Four monkeys given rhIL 11 treatment on the 13th day after irradiation had similar peripheral blood as the control group within the first 3 weeks after irradiation and recovered rapidly thereafter. The bone marrow cell culture results showed that the ability of rhIL11-treated monkeys to produce CFU Meg, CFU Mix, CFU E, BFU E and CFU GM was significantly increased in vitro. The results of histopathological examination on the 45th day after irradiation showed that the bone marrow cavity of the irradiated control group was empty and had obvious hemorrhage. In the treatment group, the cells in the bone marrow cavity of the treated group were abundant and grew vigorously. These results indicate that rhIL 11 promotes the recovery of hematopoietic progeny in irradiated macaques