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目的 研究ＰＣＲ－ ＳＳＣＰ 检测肺癌、肠癌中ｐ５３ 基因突变的方法改进及突变率。方法 用ＰＣＲ－ＳＳＣＰ，银染技术检测４ 例肺癌和７ 例大肠癌组织标本中ｐ５３ 基因外显子５ ～８ 。结果 肺癌ｐ５３ 基因突变率为７５％ （３／４ 例），肠癌ｐ５３ 基因突变率为４２．９％ （３／７ 例） 。结论 ｐ５３ 基因突变与肺癌、肠癌的发生密切相关，ＰＣＲ－ＳＳＣＰ银染技术简便快速、灵敏安全的基因突变检测方法，可应用于肿瘤的临床基因诊断。
Objective To study the improved method and mutation rate of p53 gene mutation in lung cancer and colon cancer by PCR-SSCP. Methods PCR-SSCP and silver staining techniques were used to detect exon 5～8 of p53 gene in 4 lung cancer and 7 colorectal cancer tissue samples. Results The mutation rate of p53 gene in lung cancer was 75% (3/4 cases). The mutation rate of p53 gene in colorectal cancer was 42.9% (3/7 cases). Conclusions The p53 gene mutation is closely related to the occurrence of lung cancer and colon cancer. The PCR-SSCP silver staining technique is a simple, rapid, sensitive and safe gene mutation detection method and can be applied to the clinical gene diagnosis of tumors.