基于Sol-gel膜和多壁碳纳米管/铂纳米颗粒增效的电流型L-乳酸生物传感器

• 作 者: 贺晓蕊 于京华 葛慎光 张秀明 林青 朱晗 封烁 袁靓 黄加栋
• 机 构: 济南大学化学化工学院,济南大学泉城学院,济南大学医学与生命科学学院,
• 出 处: 分析化学
• 发表日期: 2010年01期
• 关键词: 生物传感器 Sol-gel 电流型 铂纳米颗粒 溶胶-凝胶 传感器应用 检测温度 nanotubes 最佳工作条件 walled

构建了基于多壁碳纳米管(Multi-walled carbon nanotubes,MWCNTs)和铂纳米颗粒(Pt-nano)的电流型L-乳酸生物传感器。将Sol-gel膜覆盖在L-乳酸氧化酶(L-lactate oxidase,LOD)和MWCNTs/Pt-nano修饰的电极表面。实验结果表明:传感器的最佳工作条件为:检测电压0.5V;缓冲液pH6.4;检测温度25℃。此传感器的响应时间为5s,灵敏度是6.36μA/(mmol/L)。连续检测4星期其活性仍保持90%,线性范围为0.2～2.0mmol/L,且抗干扰能力强。在实际血样的检测中,此传感器与传统的分光光度法具有很好的一致性。

Current-mode L-lactic acid biosensors based on multi-walled carbon nanotubes (MWCNTs) and platinum nanoparticles (Pt-nano) were constructed. The Sol-gel membrane was coated on the surfaces of L-lactate oxidase (LOD) and MWCNTs / Pt-nano modified electrodes. The experimental results show that the optimal operating conditions of the sensor are: detection voltage 0.5V; buffer pH6.4; detection temperature 25 ℃. The sensor response time of 5s, the sensitivity is 6.36μA / (mmol / L). Continuous testing for 4 weeks its activity remained at 90%, linear range of 0.2 ~ 2.0mmol / L, and anti-interference ability. In the actual detection of blood samples, this sensor has good consistency with the traditional spectrophotometry.