论文部分内容阅读
OsPHD1基因属于水稻(Oryzasativa)植物同源结构域(PHD)-finger转录因子基因家族。逆境(干旱、高盐和低温)处理下,水稻内源基因OsPHD1的表达量明显升高。利用农杆菌介导法将OsPHD1基因转入水稻(Oryzasativassp.japonica)中花11,获得OsPHD1的过表达植株。耐逆性实验表明,过表达OsPHD1基因使转基因株系对低温(4～8℃)、高盐(200mmol/L)和干旱胁迫(相对含水量70%～95%)的耐受性分别提高43.3%、60%和25%,且在T2中获得稳定遗传。同时转基因水稻在其他性状与对照无明显差异。在洋葱(Alliumcepa)表皮亚细胞定位实验中OsPHD1与GFP的融合蛋白定位于细胞核中,qRT-PCR结果表明,OsPHD1蛋白可能通过调节胁迫反应基因的表达来调节植物的抗逆性。研究结果提示,OsPHD1基因在水稻抗逆育种上有重要的应用前景。
The OsPHD1 gene belongs to the Oryzasativa plant homolog (PHD) -finger transcription factor gene family. Under stress (drought, high salt and low temperature), the expression level of endogenous OsPHD1 gene in rice increased significantly. The OsPHD1 gene was transformed into Oryzasativassp. Japonica by flow cytometry using Agrobacterium tumefaciens-mediated method to obtain overexpression plants of OsPHD1. The results of stress tolerance test showed that over-expression of OsPHD1 increased the tolerance of transgenic lines to low temperature (4 ~ 8 ℃), high salt (200mmol / L) and drought stress (relative water content 70% ~ 95%) by 43.3 %, 60% and 25%, and obtained stable inheritance in T2. At the same time, there was no significant difference between the other traits and the control in transgenic rice. In alliumcepa epidermal subcellular localization experiment, the fusion protein of OsPHD1 and GFP was located in the nucleus. The results of qRT-PCR indicated that OsPHD1 protein may regulate plant stress resistance by regulating the expression of stress response genes. The results suggest that OsPHD1 gene has an important application prospect in rice resistance breeding.