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目的探讨自发性高血压大鼠主动脉中一氧化氮(NO)/一氧化氮合酶(NOS)体系的变化。方法随机选取健康雄性4周龄Wistar大鼠7只和4周龄自发性高血压大鼠7只,分别作为正常对照组及高血压组。4周龄及12周龄时检测二组大鼠血压。8周龄时取2组大鼠主动脉组织,采用硝酸还原酶法测定其NO水平,Western blot法检查其内皮型NOS(eNOS)以及诱导型NOS(iNOS)蛋白水平。用SPSS13.0软件对数据进行统计学分析。结果 12周龄时高血压组大鼠血压明显高于正常对照组[(24.1±0.5)kPavs(15.9±0.3)kPa](P<0.05)。12周龄时高血压组大鼠主动脉组织中NO水平较正常对照组主动脉中NO水平低[(7.2±1.7)μmol.(g prot)-1vs(17.1±5.3)μmol.(g prot)-1](P<0.05)。12周龄时高血压组大鼠主动脉中eNOS蛋白表达量与正常对照组大鼠比较差异无统计学意义[(0.5±0.2)vs(0.5±0.3)](P>0.05);12周龄时高血压组大鼠主动脉中iNOS蛋白表达量显著低于正常对照组大鼠[(0.9±0.3)vs(1.5±0.5)](P<0.05)。结论自发性高血压大鼠主动脉中NO/NOS体系下调参与高血压的形成过程。
Objective To investigate the changes of nitric oxide (NO) / nitric oxide synthase (NOS) in the aorta of spontaneously hypertensive rats. Methods Seven healthy male Wistar rats of 4 weeks old and 7 spontaneous hypertensive rats of 4 weeks were selected randomly as the normal control group and the hypertensive group. Blood pressure was measured at 4 and 12 weeks of age in both groups. At 8 weeks of age, the aortic tissues of two groups of rats were taken out. The NO level was determined by nitrate reductase method. The levels of eNOS and iNOS protein were detected by Western blot. The data were statistically analyzed with SPSS13.0 software. Results At 12 weeks of age, the blood pressure of hypertension group was significantly higher than that of the control group [(24.1 ± 0.5) kPavs (15.9 ± 0.3) kPa] (P <0.05). The level of NO in the aortic tissue of hypertensive rats at 12 weeks of age was lower than that in the normal control rats [(7.2 ± 1.7) μmol. (G prot) -1 vs (17.1 ± 5.3) μmol. (G prot) -1] (P <0.05). The expression of eNOS protein in the aorta of rats with hypertension at 12 weeks of age was not significantly different from that of the normal control rats ([(0.5 ± 0.2) vs (0.5 ± 0.3)] (P> 0.05) The expression of iNOS protein in the aorta of hypertensive rats was significantly lower than that in the normal control rats [(0.9 ± 0.3) vs (1.5 ± 0.5)] (P <0.05). Conclusion The NO / NOS system in the aorta of spontaneously hypertensive rats is down-regulated and involved in the process of hypertension.