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目的:报道30例非小细胞肺癌(NSCLC)患者放疗前后免疫功能的变化。方法:采用双抗体夹心法和比浊法检测血清中sIL2R、免疫球蛋白(IgG、IgA、IgM)含量;用单克隆抗体和流式细胞仪技术检测外周血中T细胞亚群、B细胞、NK细胞百分数;与健康人做对照。结果:放疗前sIL2R显著增高(P<0.01),IgG及IgM亦增高(P<0.05),B细胞百分数低下(P<0.01),CD3+细胞降低(P<0.01),CD4+/CD8+比值降低(P<0.05);放疗后sIL2R含量显著下降(P<0.01),IgG亦降低(P<0.05),B细胞百分数下降(P<0.05),CD3+细胞进一步下降(P<0.05),CD4+/CD8+比值亦降低(P<0.05);IgM和NK细胞放疗前后无变化。临床Ⅳ期者比Ⅱ、Ⅲ期者CD4+/CD8+比值更低(P<0.01)。结论:放射治疗会使宿主机体免疫功能降低。
Objective: To report the changes of immune function before and after radiotherapy in 30 patients with non-small cell lung cancer (NSCLC). Methods: Serum sIL2R and immunoglobulin (IgG, IgA, IgM) levels were detected by double-antibody sandwich assay and nephelometry; T cell subsets, B cells, and peripheral blood were detected by monoclonal antibodies and flow cytometry. Percentage of NK cells; contrasted with healthy subjects. Results: The sIL2R levels were significantly increased before radiotherapy (P<0.01), IgG and IgM were also increased (P<0.05), B cell percentages were low (P<0.01), CD3+ cells were decreased (P<0.01), and CD4+/CD8+ ratios were decreased (P<0.05). <0.05); sIL2R content decreased significantly after radiotherapy (P <0.01), IgG also decreased (P <0.05), B cell percentage decreased (P <0.05), CD3 + cells further decreased (P <0.05), CD4 + / CD8 + ratio also Decreased (P<0.05); IgM and NK cells did not change before and after radiotherapy. The ratio of CD4+/CD8+ was lower in patients with clinical stage IV than those in stage II and III (P<0.01). Conclusion: Radiation therapy will reduce the host’s immune function.