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血管生成在许多生理和病理过程中发挥着重要作用,但血管生成的机理仍不清楚.因此,为探明血管生成机理及开发“血管生成相关”疾病的治疗方法,在体外构建一个合适的血管生成模型是十分必要的.基于微流控系统构建了一种新型体外血管生成模型,该系统不仅能为内皮细胞生长提供一种近似于在体的微环境,并能实时监测内皮细胞对其微环境所发生变化的响应.为评价该系统用于血管生成模型建立的可行性和优越性,考察了促血管生长因子对内皮细胞增殖、迁移和管样结构形成能力的影响.研究结果表明,在促血管生长因子的诱导作用下,内皮细胞在三维基质材料中的增殖能力大大提高(提高了59.12%);在促血管生长因子浓度梯度的诱导作用下,内皮细胞定向从低浓度往高浓度侵入基质胶且形成管腔样结构.以上结果表明,该系统不仅能为血管生成机理的阐明提供一个良好的研究平台,还能为促血管生成药物或者抗血管生成药物的筛选提供一个合适的筛选平台.
Angiogenesis plays an important role in many physiological and pathological processes, but the mechanism of angiogenesis remains unclear.Therefore, in order to elucidate the mechanism of angiogenesis and to develop therapies for the development of “angiogenesis-related” diseases, a suitable in vitro Based on the microfluidic system to build a new model of in vitro angiogenesis, the system can not only provide endothelial cells with an approximate microenvironment in vivo, and real-time monitoring of endothelial cell pairs In order to evaluate the feasibility and superiority of this system in establishing angiogenesis models and to investigate the effect of pro-angiogenic growth factors on the proliferation, migration and tube-like structure formation of endothelial cells.The results show that , The proliferative ability of endothelial cells in three-dimensional matrix materials was greatly enhanced (by 59.12%) under the action of pro-angiogenic growth factor. Under the induction of the concentration gradient of vascular growth factors, the endothelial cells were oriented from low concentration to high Concentration invades the matrigel and forms a lumen-like structure.The above results show that this system not only provides an explanation of the angiogenic mechanism A good research platform, but also for the proangiogenic drugs or anti-angiogenic drug screening provides a suitable screening platform.