论文部分内容阅读
本研究对大豆病毒病抗性不同的大豆杂交亲本及F_1代,接种SMV_1号株系后的过氧化物酶和酯酶同工酶的变化进行了分析。结果表明,抗病与感病亲本及其F_1代接种后的同工酶谱带存在着明显的差异。感病亲本及F_1代酶的活性增强,酶带数较多,而且部分酶带变宽,颜色变深。抗病亲本则不发生变化。各组合F_1代的酶谱与双亲有关,抗病×抗病的F_1代酶谱与双亲完全相同,抗病×感病的F_1代酶谱偏向抗性亲本,感病×感病的F_1代产生一个双亲所不具备的小分子新杂种酶带。未接种的感病亲本酶谱与抗病亲本相同。植株内部同工酶的变化与成株抗性相一致。因此,我们认为过氧化物酶同工酶,可作为鉴定大豆抗感病毒病的生化指标之一。
In this study, the changes of peroxidase and esterase isozymes of soybean hybrids with different resistance to soybean virus disease and F 1 generation and SMV_1 inoculation were analyzed. The results showed that there was a significant difference in the isozyme bands between the resistant and the susceptible parents and their F_1 inoculation. The susceptible parents and F 1 generation of enzyme activity increased, with more enzyme bands, and part of the enzyme band widens, the color becomes darker. Disease-resistant parents did not change. The F_1 generation of each combination was related to parents, and the F_1 generation of the disease resistance × disease resistance was the same as that of the parents, and the F_1 generation of the disease resistance × susceptible F_1 generation resistant parents, susceptible × susceptible F_1 generation A parent does not have a small new hybrid enzyme band. The uninoculated susceptible parents had the same zymogram as the resistant parent. Plant isoenzyme changes and plant resistance are consistent. Therefore, we consider peroxidase isozymes as one of the biochemical markers to identify soybean anti-virus disease.