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通过对来自不同国家的50份长果黄麻种质苗期耐盐性综合评价,评估不同黄麻种质耐盐特性,筛选黄麻耐盐极端材料,为进一步挖掘黄麻耐盐基因及分子机理研究准备材料。本研究采用水培法,设0、250 mmol/L Na Cl两个浓度对50份黄麻种质材料进行处理,调查盐处理后每份材料盐害指数的变化以及第8天的死亡率,建立盐害指数随时间变化的回归模型,利用回归方程分别求出每份材料盐害症状出现的时间以及盐害指数达到50%的时间,通过主成分分析、隶属函数法、聚类分析等方法对供试材料进行综合评价和耐盐性级别划分。结果表明,250 mmol/L Na Cl胁迫对于本试验是一个适宜的浓度;建立的50个回归方程拟合效果良好;根据对50份黄麻种质材料的综合评价值及聚类分析,可以将其分为4种耐盐类型,其中,高耐盐材料3份,耐盐材料6份,盐敏感材料10份,中度耐盐材料31份。
By evaluating the salt tolerance of 50 jute germplasm from different countries at different seedling stages, evaluating the salt tolerance of different jute germplasms, selecting the jute salt tolerant extremum materials, and studying the molecular mechanism of salt tolerance for jute . In this study, 50 jute germplasm materials were treated with two concentrations of 0,250 mmol / L NaCl by hydroponic culture. The changes of salt damage index and the mortality rate on the 8th day after salt treatment were investigated to establish The regression model was used to determine the time of occurrence of salt damage symptom and the salt damage index of each material reached 50% of the time, respectively. Through principal component analysis, membership function and cluster analysis, The materials to be tested for comprehensive evaluation and salt tolerance classification. The results showed that NaCl concentration of 250 mmol / L was an appropriate concentration for this experiment. The 50 regression equations were fitted well. Based on the comprehensive evaluation of 50 jute germplasm materials and their cluster analysis, It is divided into 4 salt-tolerant types, of which 3 are high salt tolerant materials, 6 salt-tolerant materials, 10 salt-sensitive materials and 31 moderate salt-tolerant materials.