论文部分内容阅读
目的:建立和完善菥蓂药材的质量评价体系,为菥蓂的质量评价提供科学依据。方法:分别采用薄层色谱法、HPLC法,以异牡荆苷为对照品,以甲苯-甲酸乙酯-甲酸(6∶4∶1)为展开剂、10%硫酸-乙醇溶液为显色剂进行定性鉴别;以DIONEX Acclaim120-C18(4.6 mm×250 mm,5μm)色谱柱,乙腈-0.4%乙酸(20∶80)为流动相,柱温25℃,检测波长338 nm,流速0.8mL·min-1,进样量20μL,进行含量测定研究。结果:建立了菥蓂中异牡荆苷定性鉴别和含量测定方法;异牡荆苷于0.325~1.950μg(r=0.999 8)范围内呈良好的线性关系;加标回收率为99.14%(RSD为0.69%,n=6);10个不同产地菥蓂药材中异牡荆苷含量平均值为0.346 8%。结论:薄层鉴别法联合高效液相含量测定可为菥蓂药材的质量评价提供科学依据。
Objective: To establish and improve the quality appraisal system of Chinese medicinal materials so as to provide a scientific basis for the quality appraisal. Methods: TLC, HPLC, iso-vitexin as reference substance, toluene-ethyl formate-formic acid (6: 4:1) as developing solvent and 10% sulfuric acid- (4.6 mm × 250 mm, 5 μm) column with acetonitrile-0.4% acetic acid (20:80) as the mobile phase at a detection temperature of 338 nm and a flow rate of 0.8 mL · min -1, injection volume 20μL, for determination of content. Results: The method for the qualitative identification and determination of isoflavone was established. Isovitexin showed a good linearity in the range of 0.325-1.950 μg (r = 0.999 8). The spiked recoveries were 99.14% (RSD 0.69%, n = 6). The average content of iso-vitexin in 10 medicinal materials from 10 different origins was 0.346 8%. Conclusion: The TLC identification method combined with high-performance liquid content determination can provide a scientific basis for the quality evaluation of Chinese medicinal materials.