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目的:探讨腹安汤对腹泻型肠易激综合征大鼠的作用机制。方法:将48只大鼠随机分成正常组、模型组、腹安汤高、中、低剂量组及得舒特组;采用寒冷-束缚刺激法制备腹泻型肠易激综合征大鼠模型;观察给药前后大鼠的排便量,给药7天后的小肠推进功能,采用放射免疫法检测各组大鼠血浆VIP及SP含量的变化。结果:腹安汤高、中、低剂量组及得舒特组大鼠排便量减少、小肠推进运动减慢,与模型组比较差异有统计学意义(P<0.05);腹安汤高、中剂量组大鼠血浆VIP含量降低,与模型组比较有显著性差异(P<0.01);各组大鼠血浆SP含量变化均无统计学意义(P>0.05)。结论:腹安汤可抑制腹泻型肠易激综合征大鼠的小肠运动和血浆VIP的异常升高,可能是其发挥疗效的作用机制。
Objective: To investigate the mechanism of abdominal soup for diarrhea-predominant irritable bowel syndrome in rats. Methods: Forty-eight rats were randomly divided into four groups: normal group, model group, high, medium and low dosage of dian’an decoction group and DST group. The rat model of irritable bowel syndrome of diarrhea was prepared by cold-binding stimulation. The amount of defecation in rats before and after administration, small intestine propulsion after 7 days of administration, and the changes of plasma VIP and SP contents in each group were detected by radioimmunoassay. Results: Compared with model group, the rats in high, middle and low doses of Fugante Decoction had a lower amount of defecation and a slower propulsion of small intestine, the difference was statistically significant (P <0.05) Compared with the model group, the VIP level in the plasma of rats in the dose group decreased significantly (P <0.01). There was no significant difference in the content of SP in the plasma of rats in each dose group (P> 0.05). CONCLUSION: Bellyang Decoction can inhibit small intestine movement and plasma VIP in diarrhea-predominant irritable bowel syndrome rats, which may be its mechanism of action.