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Malaria is one of the most important parasitic diseases in the world which is transmitted by the Anopheles mosquitoes. There are several methods for malaria control.One of them is application of repellents on skin in malarious area as an intergrated vectro management measures.This study evaluated two repellents of DMP as a synthtic and locally product of neem oil as a botanical repellent against Anopheles stephensi.The modified method of K & D was used for the tests.Probing/biting rates on the shaved belly of white rabbits were counted. ED50 and ED95 values were calculated by probit statistic software.Calculation of effective dose(ED50, ED90 ) on human volunteer and white rabbit was performed using regression line.Protection and failure time of DMP and neem oil was also determined.ED50 value of DMP and neem oil was determined as 0.0076 and 0. 159 mg/cm respectively on white rabit.The figures of ED90 value of DMP and neem oil was determined as 0. 046 and 1.388 mg/cm~2.DMP and neem oil showed repellency effects against An.stephensi on human volunteers with ED50 value of 0.0037 and 0.127 mg/cm~2 respectively.ED90 value of DMP was determined as 0. 032 mg/cm~2.neem oil exhibited a ED90 value of 1.066 mg/cm~2 on human volunteer.The protection time of 6-7 hours for DMP and 31 minute for neem oil was determined.The failure times for DMP and Neem was 9 hours and 65 minutes rspectively on human bait.Our results exhibited that plant-based repellent is generally less effective than synthetic repellents.However,use of locally made botanical materials would be of great advantages for personal protection against mosquito biting.
Malaria is one of the most important parasitic diseases in the world which is transmitted by the Anopheles mosquitoes. There are several methods for malaria control. One of them is application of repellents on skin in malarious area as an intergrated vectro management measures. two repellents of DMP as a synthtic and locally product of neem oil as a botanical repellent against Anopheles stephensi. The modified method of K & D was used for the tests .robrobing / biting rates on the shaved belly of white rabbits were counted. ED50 and ED95 values were calculated by probit statistic software. Calculation of effective dose (ED50, ED90) on human volunteer and white rabbit was performed using regression line. Protection and failure time of DMP and neem oil was also determined. ED50 value of DMP and neem oil was determined as 0.0076 and 0. 159 mg / cm respectively on white rabit.The figures of ED90 value of DMP and neem oil were determined as 0. 046 and 1.388 mg / cm ~ 2.DMP and neem oil s howely repellency effects against An.stephensi on human volunteers with ED50 value of 0.0037 and 0.127 mg / cm ~ 2 respectively. ED90 value of DMP was determined as 0. 032 mg / cm ~ 2.neem oil exhibited a ED90 value of 1.066 mg / cm ~ 2 on human volunteer. The protection time of 6-7 hours for DMP and 31 minutes for neem oil was determined. The failure times for DMP and Neem was 9 hours and 65 minutes rspectively on human bait. Our results illustrate that plant- based repellent is generally less effective than synthetic repellents. Yet, use of locally made botanical materials would be of great advantage for personal protection against mosquito biting.