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蛋白质测定的Lowry法显色反应在30℃以下通常需30min,Larson利用加入二硫苏糖醇(DTT)使反应快速完成并提高了灵敏度,但存在DTT价格昂贵的不足。利用连二亚硫酸钠(保险粉)代替DTT,实现了蛋白质含量快速、灵敏、廉价的测定。
The Lowry method for colorimetric protein determination usually takes 30 min at below 30 ° C. Larson uses DTT to complete the reaction and increase the sensitivity, but there is a disadvantage that DTT is expensive. By using sodium dithionite instead of DTT, a fast, sensitive and inexpensive determination of protein content was achieved.