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目的 探讨血管内皮生长因子 (VEGF)抗体对骨肉瘤OS 732细胞株血管形成及瘤细胞生长状态的影响 ,为从抗血管生成途径治疗骨肉瘤提供依据。 方法 应用鸡胚绒毛尿囊膜模型 ,通过解剖显微镜下血管计数、原位铺片、组织切片HE染色及PCNA免疫组化和原位末端标记技术检测VEGF抗体对肿瘤血管形成、肿瘤生长的抑制作用及其对细胞增殖和凋亡的影响。 结果 VEGF抗体组血管密度 [给予抗体第 8天时为 (30 6± 4 6 ) ]明显低于对照组 [给予抗体第 8天时为 (5 1 0±6 9) ],瘤细胞较对照组减少 ,肿瘤细胞凋亡指数 (16 98± 4 81)显著高于PBS对照组 (3 75± 0 73) ,增殖指数两组差异不明显 ,同时VEGF抗体组凋亡的微血管内皮细胞增多 ,增殖期微血管内皮细胞少见。 结论 VEGF抗体能够显著抑制骨肉瘤OS 732血管形成 ,抑制内皮细胞增殖 ,促进内皮细胞凋亡 ,可能是VEGF抗体发挥抗血管生成作用的途径之一 ,并通过抑制血管形成而促进肿瘤细胞凋亡 ,最终达到抑制瘤细胞生长的作用。
Objective To investigate the effect of vascular endothelial growth factor (VEGF) antibody on angiogenesis and tumor cell growth in osteosarcoma OS 732 cell line and to provide a basis for anti-angiogenic therapy for osteosarcoma. Methods The chick chorioallantoic membrane model was used to detect the inhibitory effect of VEGF antibody on tumor angiogenesis and tumor growth by means of dissecting microscope blood vessel count, in situ tissue section, HE staining of tissue sections, PCNA immunohistochemistry and in situ terminal labeling And its effect on cell proliferation and apoptosis. Results The vascular density of VEGF antibody group was (30 6 ± 4 6) on the 8th day after administration of antibody, which was significantly lower than that of the control group (5 1 0 ± 6 9 after the antibody was given on the 8th day) The apoptotic index of tumor cells (16 98 ± 4 81) was significantly higher than that of PBS control group (3 75 ± 0 73), and the proliferation index was not significantly different between the two groups. At the same time, the apoptotic microvascular endothelial cells of VEGF antibody group increased, Cells rare. Conclusion VEGF antibody can significantly inhibit the angiogenesis of osteosarcoma OS 732, inhibit the proliferation of endothelial cells and promote the apoptosis of endothelial cells, which may be one of the ways of anti-angiogenesis of VEGF antibody and promote the apoptosis of tumor cells by inhibiting angiogenesis. Eventually achieve the role of inhibiting tumor cell growth.