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目的：探讨ＲＵ４８６对离体培养人早孕绒毛滋养细胞分泌ＰＡｓ及ＰＡＩ┐１、ＰＡＩ┐２的影响，比较药物流产与正常绒毛中ｔＰＡ、ｕＰＡ、ＰＡＩ┐１及ｕＰＡ┐Ｒ表达的差异。方法：应用纤维蛋白琼脂糖铺盖及反向铺盖技术分别检测细胞培养液中ＰＡ与ＰＡＩ┐１的活性，采用琼脂糖指示胶直接点样法测定ＰＡＩ┐２活性。分别应用免疫组织化学与原位杂交技术检测绒毛组织细胞中ｔＰＡ、ｕＰＡ、ＰＡＩ┐１与ｕＰＡ┐Ｒ的抗原及ｍＲＮＡ的存在与分布。结果：受ＲＵ４８６刺激，离体培养绒毛滋养细胞分泌的ＰＡ┐ＰＡＩ复合物的水解活性增加，而ＰＡＩ┐１与ＰＡＩ┐２的活性则受抑制。口服米非司酮药流绒毛滋养细胞中ＰＡＩ┐１及ｕＰＡ抗原含量低，而ｕＰＡ┐Ｒ的ｍＲＮＡ含量显著增加。结论：ＲＵ４８６增加早孕绒毛中纤溶活性，从而使细胞外基质蛋白水解作用加强，绒毛的固着性可能因此松动。
OBJECTIVE: To investigate the effect of RU486 on the secretion of PAs and PAI┐1 and PAI┐2 in human trophoblast cells cultured in vitro in early pregnancy, and to compare the differences of tPA, uPA, PAI┐1 and uPA┐R expression between medicated abortion and normal villi. Methods: The activity of PA and PAI ┐ 1 in cell culture medium was detected by using fibrin agarose blanket and reverse lapping technique. The activity of PAI ┐ 2 was determined by direct spotting method with agarose gel. Immunohistochemistry and in situ hybridization were used to detect the presence and distribution of antigens and mRNAs of tPA, uPA, PAI┐1 and uPA┐R in villus cells. Results: The RU486 stimulated the proliferation of PA┐PAI complexes secreted by cultured trophoblasts in vitro, while the activities of PAI┐1 and PAI┐2 were inhibited. Oral mifepristone abortion trophoblast trophoblast PAI ┐ 1 and uPA antigen content, uPA ┐ R mRNA content was significantly increased. CONCLUSIONS: RU486 increases fibrinolytic activity in the first trimester villi, resulting in enhanced proteolysis of the extracellular matrix, which may result in loosening of the villi.