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绵果荠是分布在新疆北部准噶尔荒漠中的十字花科一年生早春短命植物。本研究为探讨绵果荠愈伤组织诱导条件,以无菌苗子叶为外植体,研究了不同苗龄、培养基种类、蔗糖浓度和培养条件对愈伤组织的诱导及其生长的影响。结果表明,1)愈伤组织诱导的最佳条件为:苗龄14d、培养基为MS+1.0mg/L IBA+0.1mg/L BAP、含糖量3%、温度25℃和光照为黑暗。2)愈伤组织生长的适宜条件是光照16h/d和温度25℃。
Water chestnuts are distributed in the northern part of Junggar desert in northern cruciferous early spring ephemeral plants. In this study, we investigated the callus induction conditions and took aseptic seedling leaves as explants to study the effects of different seedling age, medium type, sucrose concentration and culture conditions on callus induction and growth. The results showed that: 1) The optimal conditions for callus induction were as follows: seedling age 14d, medium MS + 1.0mg / L IBA + 0.1mg / L BAP, sugar content 3%, temperature 25 ℃ and light darkness. 2) Suitable conditions for callus growth are light 16h / d and temperature 25 ℃.