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目的：探讨Ｔ淋巴细胞是否参与了异种肝移植免疫排斥反应。方法：建立仓鼠到大鼠原位肝移植模型，用免疫组织化学染色方法检测异种肝移植免疫排斥反应中Ｔ淋巴细胞浸润情况及其Ｆａｓ抗原配体（Ｆａｓ－Ｌ）的表达，并用普通组织学及原位末端标记方法检测移植肝中细胞凋亡。结果：异种肝移植免疫排斥过程中，移植肝中Ｔ淋巴细胞，包括ＣＤ４，ＣＤ８亚型大量浸润，术后第４天开始表达Ｆａｓ－Ｌ，随着排斥反应的加重，Ｆａｓ－Ｌ表达越高，细胞凋亡数量也越多。结论：Ｔ淋巴细胞参与了异种肝移植免疫排斥反应。
Objective: To investigate whether T lymphocytes participate in the immune rejection of xenograft liver transplantation. Methods: Hamster-to-rat orthotopic liver transplantation was established. The infiltration of T lymphocytes and the expression of Fas-Ligand (Fas-L) in the rejection of xenotransplantation liver transplantation were detected by immunohistochemical staining. And in situ terminal labeling method to detect apoptosis in transplanted liver. Results: During the rejection of xenotransplantation, T lymphocytes, including CD4 and CD8 subtypes in the transplanted liver were extensively infiltrated, Fas-L began to express on the 4th day after transplantation, and the expression of Fas-L was increased with the increase of rejection , The more the number of apoptosis. Conclusion: T lymphocytes participate in the immune rejection of xenotransplantation of liver.