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作者从内蒙古锡林郭勒草原优质牧草-羊草（Leymuschinensis）根际分离筛选出两株根际联合固氮菌。根据形态特征和生理生化特性，菌株A－1属催娩克雷伯氏菌（Klebsiellaoxytoca），菌株B－18属肺炎克雷伯氏菌肺炎亚种（Klebsiellapneumoniaesubsp.pneumoniae）。对其进行生理生化特性、培养特性、固氮酶活性、氧浓度对固氮酶活性影响等方面的研究。结果表明，菌株均在微好氧条件下固氮活性最高，在Hill无氮培养基上30℃恒温培养，其固氮酶活性分别在18和16小时左右达到最高峰。
The authors isolated and isolated two rhizosphere azotobacter strains from the rhizosphere of Leymus chinensis, a high-quality pasture in Xilin Gol Grassland, Inner Mongolia. According to the morphological characteristics and physiological and biochemical characteristics, strain A-1 belongs to Klebsiella oxytoca and strain B-18 belongs to Klebsiella pneumoniae subsp. Pneumoniae. The physiological and biochemical characteristics, culture characteristics, nitrogenase activity, oxygen concentration on nitrogenase activity and other aspects of the study. The results showed that all the strains had the highest nitrogen fixation activity under micro-aerobic conditions. The strains were incubated in Hill nitrogen-free medium at 30 ° C, and their nitrogenase activity peaked at around 18 and 16 hours respectively.