论文部分内容阅读
目的通过顺铂联合氟西汀对肺癌伴抑郁小鼠进行干预,检测相关指标,观察氟西汀能否通过抗抑郁作用对肿瘤起到抑制作用。方法通过社交失败应激及皮下接种Lewis肺癌细胞株建立小鼠肺癌伴抑郁模型;ELISA方法检测血清皮质醇及IL-6水平;RT-PCR方法检测海马组织中脑源性神经营养因子(brain derived neurotropic factor,BDNF)mRNA表达;免疫组织化学方法检测肿瘤组织核转录因子-κB(nuclear factor-κB,NF-κB)及血管内皮生长因子(vascular endothelial growth factor,VEGF)蛋白表达。结果双药组皮质醇与IL-6水平与模型组相比明显降低(P<0.05);双药组海马BDNF mRNA表达显著升高(P<0.05);双药组肿瘤重量、肿瘤组织中NF-κB及VEGF蛋白表达水平与单药组相比差异无显著性(P>0.05)。结论氟西汀能够降低肺癌伴抑郁小鼠血清皮质醇水平并促进海马组织BDNF mRNA表达而发挥抗抑郁作用,但并未发现其抗抑郁效果能够抑制肿瘤进展。
Objective To investigate the effect of fluoxetine on tumor progression through intervention of cisplatin and fluoxetine in mice with lung cancer and depression. Methods The mouse model of lung cancer with depression was established through social failure and subcutaneous inoculation of Lewis lung cancer cell lines. Serum cortisol and IL-6 levels were measured by ELISA. The brain derived neurotrophic factor (BDNF) , neurotrophic factor (BDNF) mRNA expression were detected by immunohistochemistry. The expression of NF-κB and VEGF protein in tumor tissue were detected by immunohistochemistry. Results The levels of cortisol and IL-6 in the two groups were significantly lower than those in the untreated group (P <0.05). BDNF mRNA expression in the hippocampus was significantly increased in the two groups (P <0.05) There was no significant difference in the expression of -κB and VEGF between the two groups (P> 0.05). Conclusion Fluoxetine can reduce the level of serum cortisol and promote the expression of BDNF mRNA in the hippocampus of lung cancer patients. However, no effect of antidepressant on tumor progression was found.