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目的 :检测高迁徙率族蛋白1(high mobility group protein 1,HMGB1)及其受体晚期糖基化终末产物受体(receptor for advanced glycation end-products,RAGE)在肝内胆管癌组织中的表达;探讨HMGB1/RAGE对肝内胆管癌细胞增殖及上皮-间质细胞转化(epithelial-mesenchymal transition,EMT)过程中的作用。方法:免疫组化分析未转移和已转移胆管癌患者标本中HMGB1及RAGE的表达;ELISA法检测胆管癌及胆管结石患者胆汁中HMGB1的含量;CCK8、Transwell检测HMGB1及RAGE抑制剂(FFP-ZM1)对胆管癌细胞增殖、侵袭作用的影响;Western blot检测胆管癌及癌旁组织中p-ERK的表达。结果:HMGB1和RAGE在已转移的胆管癌患者组织标本中高表达,HMGB1可促进胆管癌细胞的EMT过程及胆管癌细胞的生长和侵袭。结论 :HMGB1可参与胆管癌细胞的增殖与EMT过程。
AIM: To detect the expression of high mobility group protein 1 (HMGB1) and its receptor for advanced glycation end-products (RAGE) in intrahepatic cholangiocarcinoma To investigate the role of HMGB1 / RAGE in the proliferation and epithelial-mesenchymal transition (EMT) of intrahepatic cholangiocarcinoma cells. Methods: The expression of HMGB1 and RAGE in specimens of patients with metastasized and metastasized cholangiocarcinoma was analyzed by immunohistochemistry. The content of HMGB1 in bile of patients with cholangiocarcinoma and bile duct stones was detected by ELISA. The expressions of HMGB1 and RAGE inhibitor (FFP-ZM1) ) On the proliferation and invasion of cholangiocarcinoma cells. Western blot was used to detect the expression of p-ERK in cholangiocarcinoma and paracancerous tissues. Results: HMGB1 and RAGE were highly expressed in the tissue samples of patients with metastatic cholangiocarcinoma. HMGB1 could promote the EMT process of cholangiocarcinoma cells and the growth and invasion of cholangiocarcinoma cells. Conclusion: HMGB1 can participate in the proliferation and EMT of cholangiocarcinoma cells.