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目的：探讨单一及多位点核酶对乙型肝炎病毒靶RNA的切割作用．方法：利用计算机辅助设计针对乙型肝炎病毒C基因的三个单一核酶及特异联合型核酶，构建三个核酶各自及三个核酶串联的自剪切转录载体（pGEMRz123），观察单一及串联核酶（Rz123）对靶RNA的切割作用．结果：构建的串联核酶自剪切转录载体体外转录后，在顺式核酶发生自剪切后可将目的核酶正确地释放出来，计算机设计的单一核酶体外可剪切靶RNA；串联核酶中的单一核酶不仅可单独作用，而且可联合切割，提高了单一核酶的剪切效率．结论：抗乙型肝炎病毒特异联合型核酶体外可联合切割靶RNA.
Objective: To investigate the cleavage effect of single and multi-site ribozymes on target RNA of hepatitis B virus. Methods: Three single ribozymes against hepatitis C virus C and three specific ribozymes were designed by computer to construct a self-cleaving transcription vector (pGEMRz123) And tandem ribozyme (Rz123) on the target RNA cleavage. Results: The constructed ribozyme was transcribed from the transcribed vector in vitro. After the ribozyme was self-sheared, the target ribozyme could be correctly released. The single ribozyme designed by the computer could cut the target RNA in vitro. Ribozymes in a single ribozyme not only can act alone, but also combined cleavage, improve the cleavage efficiency of a single ribozyme. Conclusion: The anti-HBV specific ribozyme can cut target RNA in vitro.