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本文以人低分化鼻咽癌(NPC)上皮细胞系(CNE-2Z)为对象,用蛋白激酶C(PKC)三种主要亚型的单克隆抗体,采用点印迹(Dot Blotting)法检测细胞颗粒部分与胞浆部分PKC各亚型的含量及分布.采用MTT
In this study, human poorly-differentiated nasopharyngeal carcinoma (NPC) epithelial cell line (CNE-2Z) was used as a target, and monoclonal antibodies against three major subtypes of protein kinase C (PKC) were used to detect cell granules using dot blotting. Partial and cytoplasmic fractions of PKC subtypes and their distribution.