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本研究对细胞质雄性不育“三系”和细胞核雄性不育系的种子及不同发育时期的花药进行了酯酶同工酶、过氧化物酶同工酶,游离脯氨酸含量和过氧化物酶活性的测定与分析。结果表明:(1)种子酯酶同工酶在细胞质雄性不育“三系”之间表现出明显差异,酶谱遗传分析表明,1E,2E酶带与育性恢复有关,8E,9E酶带与不育有关,(2)不同发育时期过氧化物酶同工酶酶带数不育株(系)均少于可育株(系);(3)花药游离脯氯酸含量可育阳(系)明显高于不育株(系);(4)随着小孢子发育进展,细胞核雄性不育株过氧化物酶活性急剧增长,其增长速度高出可育株几倍。过氧化物酶同工酶酶带减少和酶活性在小孢子发育过程中的急剧增高,被认为是引起花粉败育的重要原因,本文还提出了在“三系”选育过程中结合同工酶分析技术加速细胞质雄性不育恢复系选育进程的设想。
In this study, anther isozymes, peroxidase isoenzymes, free proline content and peroxides in three-line and cytoplasmic male sterile lines of seeds and anther of different developmental stages were studied. Determination and analysis of enzyme activity. The results showed that: (1) Seeds esterase isozymes showed obvious difference among the “three lines” of cytoplasmic male sterility. The genetic analysis of the zymogram showed that the bands of 1E and 2E were related to the restoration of fertility. The bands of 8E and 9E (2) The numbers of peroxidase isozymes in different developmental stages were less than that in fertile plants (lines); (3) The content of free proline in anthers was Line) was significantly higher than that of sterile lines (lines). (4) With the development of microspore, the peroxidase activity of nuclear male sterile lines increased sharply, which was several times higher than that of fertile lines. The decrease of peroxidase isozymes and the rapid increase of enzyme activity during microspore development are considered as the important reasons for pollen abortion. In this paper, Assumption of Enzyme Assay to Accelerate Breeding Process of Cytoplasmic Male Sterility Restorer Line.