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用细胞培养及trkA免疫组化染色方法，观察了坐骨神经外植块及巨噬细胞和雪旺氏细胞条件培养基对胚鼠脊神经节感觉神经元高亲和力神经生长因子受体表达的影响。结果显示：各组感觉神经元均产生免疫阳性反应，雪旺氏细胞条件培养基和巨噬细胞条件培养组免疫反应强度与对照组相同，联合培养组免疫反应为强阳性，与巨噬细胞条件培养组相比差别高度显著（P＜0.005）。免疫反应阳性神经元可分为2类：一类形状为圆形或椭圆形，另一类为多角形。实验组神经元和非神经元生长状况明显优于对照组。本研究结果提示：坐骨神经的溃变轴突有促进脊神经节内感觉神经元trkA表达的作用;雪旺细胞条件培养和巨噬细胞条件培养组的某些因子可直接或间接地维持感觉神经元的生存，但不促进其trkA的表达。
The effects of sciatic nerve explants, macrophages and Schwann cells conditioned medium on the expression of high affinity nerve growth factor receptor in the sensory neurons of the dorsal root ganglion of the embryo were observed by cell culture and trkA immunohistochemical staining. The results showed that: the sensory neurons in each group had an immunopositive reaction, and the immunoreactivity in the conditions of Schwann cell conditioned medium and macrophage conditioned medium was the same as that in the control group. The immunophenotype in the co-culture group was strongly positive, The difference between the groups was highly significant (P <0.005). Immunoreactive neurons can be divided into two categories: one is round or oval, the other is polygonal. Experimental group of neurons and non-neuronal growth was significantly better than the control group. Our results suggest that the degenerative axons of the sciatic nerve promote the expression of trkA in sensory neurons in spinal ganglia. Some factors in Schwann cell culture and macrophage conditioned culture may directly or indirectly maintain the expression of sensory neurons Survival, but did not promote its trkA expression.