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采用直接免疫荧光技术研究肾虚、脾虚和脾胃湿热患者的细胞免疫功能,结果表明:3组患者的CD3+、CD4+细胞低于健康人(P<0.01);脾虚、脾胃湿热患者的CD4+/CD8+比值低于健康人(P<0.01),而肾虚患者却高于健康人(P<0.01)。用免疫灵合剂治疗30例肾虚患者,结果表明肾虚患者治疗后的CD3+、CD4+细胞较治疗前明显增加(P<0.01),说明补肾治疗能增强肾虚患者的细胞免疫功能。CD4+/CD8+比值能指导中医临床辨证分型和评价治疗效果。
The direct immunofluorescence technique was used to study the cellular immune function in patients with kidney deficiency, spleen deficiency and spleen and stomach dampness fever. The results showed that the CD3+ and CD4+ cells in the three groups were lower than those in healthy people (P<0.01); CD4+/CD8+ in patients with spleen deficiency and spleen and stomach dampness. The ratio was lower than healthy people (P<0.01), but the patients with kidney deficiency were higher than healthy people (P<0.01). Treatment of 30 patients with kidney deficiency with immunosuppressant agent showed that the number of CD3+ and CD4+ cells in patients with kidney deficiency after treatment was significantly higher than before treatment (P<0.01), indicating that Bushen treatment can enhance the cellular immune function in patients with kidney deficiency. The CD4+/CD8+ ratio can guide clinical syndrome differentiation and evaluate the treatment effect.