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观察扶正化瘀方药物血清体外给药对正常大鼠肝星状细胞,及该方体内给药对二甲基亚硝胺纤维肝星状细胞胶原生成与表达的影响。一、材料与方法Wistar 雄性大鼠。扶正化瘀方制成每克含生药2.703克的流浸膏。正常大鼠以0.46g/kg 体重剂量扶正化瘀方浸膏稀释液灌胃,第一次给药2小时后相同剂量重复给药一次,再1小时后,后腔静脉采血,离心、灭活以制备药物血清。二甲基亚硝胺腹腔注射制备肝纤维化大鼠模型,扶正化瘀方同上剂量治疗7天。按本所常规方法分离纤维肝及正常肝大鼠星状细胞,正常星状细胞传一代培养,药物血清温育72小时,纤维肝星状细胞原代培养48小时。脯氨酸掺入、胶原酶消化法测定细胞内、外胶原生成率。细胞免疫化学 PAP 法染色Ⅰ、Ⅲ、Ⅳ型胶原。统计方法采用验验。二、结果
Observe the effect of Fuzheng Huayu prescription drug serum on hepatic stellate cells in normal rats and the effects of this prescription on collagen production and expression in dimethylnitrosamine fiber hepatic stellate cells. I. Materials and Methods Wistar male rats. The Fuzheng Huayufang was made into a flow extract containing 2.703 grams of crude drug per gram. The normal rats were intragastrically administered with 0.46g/kg body weight of Fuzheng Huayufang extract diluent. The same dose was repeated once every 2 hours after the first administration. After 1 hour, blood was collected from the vena cava, centrifuged, and inactivated. To prepare drug serum. A rat model of hepatic fibrosis was prepared by intraperitoneal injection of dimethylnitrosamine and Fuzheng Huayu Recipe was treated with the same dose as above for 7 days. The stellate cells of fibrotic liver and normal liver were isolated according to the conventional method of the Institute. Normal stellate cells were cultured for one generation, drug serum was incubated for 72 hours, and fibrotic hepatic stellate cells were cultured for 48 hours. Proline incorporation and collagenase digestion were used to determine intracellular and extracellular collagen production. Cellular immunochemistry PAP staining for type I, III, IV collagen. The statistical method uses inspection. Second, the results