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目的为进一步研究天然抗氧化剂茶多酚（Ｔｅａｐｏｌｙｐｈｅｎｏｌｓ，ＴＰ）的心血管和神经系统作用，观察了ＴＰ对离体大鼠工作心脏心功能、培养的大鼠交感神经细胞钙内向电流和小鼠脑能量代谢的影响。方法①在灌流液中加入ＴＰ（１ｍｇ／Ｌ），比较给药前后离体大鼠工作心脏心功能的改变；②用膜片钳全细胞记录技术观察ＴＰ对培养交感神经细胞Ｃａ２＋内向电流的影响；③用３１Ｐ核磁共振（ＮＭＲ）技术观察ＴＰ给药后小鼠脑能量物质的变化。结果①ＴＰ使离体大鼠工作心脏的主动脉流量、左室收缩压和最大上升速度明显增加；②ＴＰ明显增加交感神经细胞的钙内向电流；③ＴＰ可以调节小鼠脑细胞能量代谢，特别是明显增加脑中磷酸肌酸的含量。结论实验进一步证实了ＴＰ的强心作用，其作用机理可能与激活细胞钙通道有关。此外，ＴＰ有助于脑细胞的能量代谢。
Objective To further investigate the cardiovascular and neurological effects of natural antioxidants Teapolyphenols (TP), the effects of TP on cardiac cardiac function in isolated rat hearts, inducible calcium currents in rat sympathetic cells and in mouse brain The impact of energy metabolism. Methods ① TP (1 mg / L) was added into the perfusate to compare the changes of cardiac function in isolated rat hearts before and after administration. ② The effect of TP on the inward current of Ca2 + in sympathetic neurons was observed by patch clamp whole cell recording ; ③ The 31P nuclear magnetic resonance (NMR) technique was used to observe the changes of brain energy and substance in TP mice. Results ①TP significantly increased aortic flow, left ventricular systolic pressure and maximal ascending velocity in isolated working heart; ②TP significantly increased calcium inward current of sympathetic cells; ③TP could regulate the energy metabolism of mouse brain cells, especially markedly increased Phosphocreatine brain content. Conclusions The experiment further confirmed the cardiac effect of TP, which may be related to the activation of cellular calcium channels. In addition, TP contributes to the energy metabolism of brain cells.