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我们应用几种形态学方法观察大鼠颌下腺缺血及再灌注不同时相上皮细胞的凋亡与增殖特征 ,以揭示缺血再灌注颌下腺组织损伤与修复过程中细胞凋亡和增殖与颌下腺上皮细胞丧失的关系。一、材料和方法1.材料 :增殖细胞核抗原 (ＰＣＮＡ)单克隆抗体、免疫组织化学染色ＳＦＴＭ试剂盒
We used several morphological methods to observe the submandibular gland ischemia and reperfusion in different phases of epithelial cell apoptosis and proliferation characteristics in order to reveal the process of ischemia-reperfusion submandibular gland tissue damage and repair apoptosis and proliferation and submandibular gland epithelial cells Loss of relationship. First, the material and method 1 material: proliferating cell nuclear antigen (PCNA) monoclonal antibody, immunohistochemical staining SFTM kit