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AIM: To test the effect of the dephytinization of three different commercial infant cereals on iron, calcium,and zinc bioavailability by estimating the uptake, retention,and transport by Caco-2 cells.METHODS: Both dephytinized (by adding an exogenous phytase) and non-dephytinized infant cereals were digested using an in vitro digestion protocol adapted to the gastrointestinal conditions of infants younger than 6 mo. Mineral cell retention, transport,and uptake from infant cereals were measured using the soluble fraction of the simulated digestion and the Caco-2 cells.RESULTS: Dephytinization of infant cereals significantly increased ( P < 0.05) the cell uptake efficiency (from 0.66%-6.05% to 3.93%-13%), retention (from 6.04%-16.68% to 14.75%-20.14%) and transport efficiency (from 0.14%-2.21% to 1.47%-6.02%), of iron, and the uptake efficiency (from 5.0%-35.4% to 7.3%-41.6%) and retention (from 4.05%-20.53% to 14.45%-61.3%) of zinc, whereas calcium only cell uptake showed a significant increase ( P < 0.05) after removing phytate from most of the samples analyzed.A positive relationship ( P < 0.05) between mineral solubility and the cell uptake and transport efficiencies was observed.