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为研究ＣＤ１３７在正常人静止状态和活化Ｔ细胞上的表达特点及ＣＤ１３７单抗协同ＰＨＡ 刺激Ｔ细胞增殖作用。利用流式细胞术测定ＣＤ１３７的表达，利用３Ｈ－ＴｄＲ掺入法测定Ｔ细胞增殖。结果显示：静止Ｔ细胞上ＣＤ１３７表达率为（０．６５±０．０５）％ ，经ＰＨＡ 刺激后２４、４８、７２ｈ，Ｔ 细胞上ＣＤ１３７表达率分别为（８．１６±１．２８）％ 、（１２．３９±２．１５）％ 、（２１．６±４．３６）％ 。ＣＤ＋４ Ｔ细胞和ＣＤ＋８ Ｔ细胞均可表达ＣＤ１３７，且ＣＤ＋４ Ｔ细胞的表达率高于ＣＤ＋８ Ｔ细胞。单独用ＣＤ１３７单抗对Ｔ细胞的刺激指数（ＳＩ）为１．０４，单独用ＰＨＡ 刺激指数为２．３１，不同浓度ＣＤ１３７单抗（１、５、１０）ｍ ｇ／Ｌ和ＰＨＡ 共同作用刺激指数分别为２．８３、４．２５、５．７４。ＣＤ１３７ＭｃＡｂ（１０ｍ ｇ／Ｌ）＋ ＰＨＡ 组刺激指数比其他各组均明显增高（Ｐ＜ ０．０５），并随ＣＤ１３７ＭｃＡｂ 浓度增高对Ｔ 细胞的增殖逐渐增高。表明静止Ｔ细胞上不表达ＣＤ１３７，Ｔ细胞活化后，ＣＤ１３７表达量逐渐增加。ＣＤ１３７单抗可显著增加ＰＨＡ对Ｔ细胞的增殖作用，而在无ＰＨＡ 时，ＣＤ１３７单抗对Ｔ细胞无增殖作用
To study the expression of CD137 on quiescent human normal and activated T cells, CD137 monoclonal antibody synergizes with PHA to stimulate T cell proliferation. CD137 expression was measured by flow cytometry and T cell proliferation was measured by 3H-TdR incorporation. The results showed that the expression rate of CD137 on resting T cells was (0.65 ± 0.05)%, the positive rate of CD137 on T cells was (8.16 ± 1.28)% at 24, 48 and 72 h after PHA stimulation, , (12.39 ± 2.15)%, (21.6 ± 4.36)%. CD137 was expressed on both CD + 4 T cells and CD + 8 T cells, and the expression of CD + 4 T cells was higher than that of CD + 8 T cells. The stimulation index (SI) of T cells by CD137 alone was 1.04, the stimulation index by PHA alone was 2.31, and stimulation with different concentration of CD137 monoclonal antibody (1,5,10) m g / L and PHA The indices were 2.83, 4.25 and 5.74 respectively. The stimulation index of CD137McAb (10m g / L) + PHA group was significantly higher than that of other groups (P <0.05), and the proliferation of T cells was gradually increased with the increase of CD137McAb concentration. CD137 showed no expression of resting T cells, T cell activation, CD137 expression gradually increased. CD137 mAb significantly increased the proliferative effect of PHA on T cells, while in the absence of PHA, CD137 mAb did not proliferate on T cells