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目的建立准确、灵敏、可靠的LC-MS/MS法,定量测定莫达非尼对映体,考察其在Caco-2细胞单层模型上的转运。方法选用资生堂C8(50 mm×2.1 mm,3μm)色谱柱及API4000液相色谱-质谱联用仪,用于定量分析的离子反应分别为m/z 274.4→165.1(莫达非尼)和m/z 391.2→265.1(CS55)。结果在本实验条件下,建立范围为1～1000 ng/ml的定量方法,批内、批间精密度<15%(n=5)。结论建立一种快速、准确、灵敏度高、专属性好,可用于莫达非尼对映体在Caco-2单层细胞模型转运研究的方法。
OBJECTIVE To establish an accurate, sensitive and reliable LC-MS / MS method for quantitative determination of modafinil enantiomers and investigate their transport in monolayers of Caco-2 cells. Methods Ion exchange reactions for multivariate analysis of m / z 274.4 → 165.1 (modafinil) and m / z were performed on a Shimadzu C8 (50 mm × 2.1 mm, 3 μm) column and an API4000 liquid chromatography- z 391.2 → 265.1 (CS55). Results Under the experimental conditions, a quantitative method with a range of 1 ~ 1000 ng / ml was established. The intra- and inter-assay precision was <15% (n = 5). Conclusion A rapid, accurate, sensitive and specific method for the determination of modafinil enantiomers in Caco-2 monolayer cells was established.