Construction of Prokaryotic Expression Vector for At4g12500 Gene of Arabidopsis

• 作 者: Yuan YU Lan LI
• 机 构: DepartmentofBiochemistryandMolecularBiology,DepartmentofScientificResearch
• 出 处: 农业生物技术：英文版
• 发表日期: 2012年4期
• 关键词: 基因组DNA提取 原核表达载体 拟南芥 大肠杆菌表达 PCR技术 DOPS 阿拉伯 野生型 Atdg12500 gene Vector constructio

[Objective] This study aimed to construct the prokaryotic expression vector for Arabidopsis At4g12500 gene. [Method] Genomic DNA of wild-type Arabidopsis Col-0 was extracted as the template for amplification of At4g12500 gene with PCR technology. The targ