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目的 建立多色荧光原位杂交技术检测人卵细胞染色体非整倍体的方法。方法 取试管婴儿助孕技术后未能受精成功的卵细胞 ,于取卵后 1～ 3d固定 ,采用多色荧光原位杂交方法检测卵细胞 13,16 ,18,2 1和 2 2号染色体的情况。结果 正常未受精卵细胞中期染色体显示一个成对的杂交信号 ,每条染色单体显示一个单个信号 ;分裂相中多出或缺少一个成对杂交信号表明多余或缺少一条染色体 ;分裂相中多出或缺少一个单个信号表明多余或缺少一条染色单体 ;两个单个信号分离表明两条姐妹染色单体分离。结论 采用多色荧光原位杂交方法可以有效检测人卵细胞染色体非整倍体异常。
Objective To establish a multi-color fluorescence in situ hybridization method for detecting human oocyte chromosome aneuploidy. Methods Egg cells that failed to fertilize after in vitro fertilization with IVF were fixed 1 to 3 days after oocyte retrieval. The chromosomes 13, 16, 18, 21 and 22 were detected by multicolor fluorescence in situ hybridization. Results The normal metaphase chromosomes of unfertilized ova showed one pair of hybridization signals, each of which showed a single signal; the excess or absence of one pair of hybridization signals in the split phase indicated the excess or lack of one chromosome; The absence of a single signal indicates excess or absence of a single chromatid; separation of two single signals indicates separation of two sister chromatids. Conclusion Multi-color fluorescence in situ hybridization can effectively detect human aneuploidy aneuploidy.