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目的 通过对血清中与癌症相关分子标志物的荧光光谱分析 ,探讨在分子、电子水平上对恶性肿瘤的筛查方法。方法 采取肘静脉血并制备血清 ,采用光致发光的方法研究血清中的发光中心 ,观察机体产生恶性肿瘤后原卟啉、类胡萝卜素代谢的变化。通过经原卟啉、类胡萝卜素相对含量测量归纳出的经验函数 ,施行恶性肿瘤的临床诊断。结果 血清中主要的发光分子是蛋白质、类胡萝卜素、卟啉等。观察到癌患者的卟啉、类胡萝卜素代谢异常。在早期阶段随着肿瘤的发展癌患者血液中的卟啉水平升高 ;在进展期随着肿瘤的发展癌患者血液中的卟啉水平逐渐下降而类胡萝卜素代谢在升高。采用Ⅰ =ⅠA ⅠB - 1的经验公式开展了恶性肿瘤的临床检测 ,其诊断符合率达 90 %。结论 癌患者卟啉代谢异常 ,原卟啉Ⅸ可以作为癌的分子标志物。通过血清荧光分析进行原卟啉Ⅸ的相对测量 ,采用归纳出的经验函数 ,能够施行恶性肿瘤的临床筛查
Objective To investigate the screening methods of malignant tumors at the molecular and electronic levels by analyzing the fluorescence spectra of serum-associated molecular markers. Methods The elbow venous blood was taken and serum was prepared. The luminescence center in serum was studied by photoluminescence, and the changes of protoporphyrin and carotenoid metabolism were observed after malignant tumors were produced. Through the protoporphyrin, the relative content of carotenoids measurement derived from the empirical function, the implementation of the clinical diagnosis of malignant tumors. Results The main luminescent molecules in serum were protein, carotenoid, porphyrin and so on. Porphyrin and carotenoid metabolism were observed in cancer patients. In the early stages as cancer progresses, the level of porphyrins in the blood of patients with cancer increases; in the advanced stages of cancer, the level of porphyrins in the blood of cancer patients declines and carotenoid metabolism is increased. The clinical test of malignant tumor was carried out using the empirical formula of Ⅰ = ⅠA ⅠB - 1, and its diagnostic coincidence rate reached 90%. Conclusions Porphyrin metabolism is abnormal in cancer patients and protoporphyrin Ⅸ can be used as molecular marker of cancer. The relative measurement of protoporphyrin IX by serum fluorescence analysis and the use of summarized empirical functions allow the clinical screening of malignant tumors