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目的 研究氦氖激光血管内照射 (ILIB)对肿瘤浸润淋巴细胞 (TIL)生物活性的影响。方法 ILIB组 6 0例恶性肿瘤患者用ILIB加生物治疗 ,对照组 6 0例恶性肿瘤患者用模拟照射加生物治疗 ,分别测定照射前后TIL细胞增殖活性、细胞亚群 (APAAP法 )、细胞毒性 (MTT法 )与端粒酶活性 (TRAP -PCR法 )。结果 ILIB组治疗 2 0d后TIL增殖活性与对照组及治疗前相比 ,差异无显著性 (P >0 .0 5 ) ;CD3、CD4、CD4/CD8明显高于治疗前及对照组 (P <0 .0 5 ) ,而CD8差异无显著性 (P >0 .0 5 ) ,TIL细胞毒性治疗后 (4 1.0± 6 .2 ) %明显高于治疗前 (2 1.1± 4.0 ) %及对照组 (2 5 .1± 3 .1) %(P <0 .0 1) ;TIL端粒酶活性治疗后 0 .0 6 1± 0 .0 0 1明显高于治疗前 0 .0 2 8± 0 .0 0 9及对照组 0 .0 2 5±0 .0 0 7(P <0 .0 5 )。ILIB组缓解率 5 3 %、有效率 79% ,明显高于对照组缓解率 2 8% (χ2 =7.84,P <0 .0 1)、有效率 48% (χ2 =12 .17,P <0 .0 0 1)。结论 ILIB引起TILCD3、CD4、CD4/CD8、细胞毒性与端粒酶活性增加 ,增加肿瘤患者的免疫功能 ,增强TIL抗肿瘤效应 ,ILIB辅助生物治疗有希望进一步提高恶性肿瘤患者生存率。
Objective To investigate the effect of He-Ne laser intravascular irradiation (ILIB) on the biological activity of tumor-infiltrating lymphocytes (TILs). Methods Sixty patients with malignant tumor in ILIB group were treated with ILIB plus biotherapy. Sixty patients in control group were treated with simulated irradiation and biologic therapy. TIL cell proliferative activity, cell subpopulation (APAAP), cytotoxicity MTT method) and telomerase activity (TRAP-PCR method). Results Compared with the control group and before treatment, the proliferative activity of TIL in ILIB group was not significantly different after 20 days (P> 0.05). The levels of CD3, CD4 and CD4 / CD8 in ILIB group were significantly higher than those before treatment and control group (P < (P <0.05), but the difference of CD8 was not significant (P> 0.05). After TIL cytotoxicity treatment (4.01 ± 6.2%) was significantly higher than that before treatment (1.1 ± 4.0)% and control group (25.1 ± 3.1)% (P <0.01). The telomerase activity of TIL after treatment was significantly higher than that before treatment (0.066 ± 0.001) .0 0 9 and control group 0 .0 2 5 ± 0 .0 0 7 (P <0. 05). ILIB group had a response rate of 53% and an effective rate of 79%, which was significantly higher than that of the control group (χ2 = 7.84, P <0.01), and the effective rate was 48% (χ2 = 12.17, P <0 .0 0 1). CONCLUSION ILIB induces increased TILCD3, CD4, CD4 / CD8, increased cytotoxicity and telomerase activity, increased immune function and enhanced anti-tumor effect of TIL. ILIB-assisted biotherapy may further improve the survival rate of patients with malignant tumor.