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目的:探讨血管紧张素转换酶(ACE)基因多态性在结直肠癌(CRC)发病中的作用。方法:入组120例CRC患者,采用聚合酶链反应(PCR)检测患者外周血中ACE基因多态性和等位基因分布频率,研究ACE基因缺失型(DD型)、II型和插入型(ID型)等3种基因型及I/D等位基因在CRC患者外周血中的分布频率,并与120例正常人检测结果进行对比。结果:CRC患者外周血标本中存在ACE基因插入/缺失(I/D)多态性分布,DD型、ID型和II型3种基因型的频率分别为15.00%,50.00%,35.00%,I/D等位基因分布频率分别为60.00%及40.00%;对照组的分布频率分别为16.67%,53.33%,30.00%,I/D等位基因分布频率分别为56.67%及43.33%,两组间3种基因型的分布频率无显著性差异(P>0.05)、I/D等位基因分布频率无显著差异(P>0.05)。分层分析表明,D等位基因变异与CRC低分化和远处转移相关(P<0.01及P<0.05)。结论:ACE基因I/D多态性与CRC存在一定关系,其中D等位基因异常与CRC低分化和易转移可能有关。
Objective: To investigate the role of angiotensin converting enzyme (ACE) gene polymorphism in the pathogenesis of colorectal cancer (CRC). Methods: One hundred and twenty patients with CRC were enrolled in this study. ACE gene polymorphism and allele frequencies in peripheral blood were detected by polymerase chain reaction (PCR). The relationship between ACE gene deletion type (DD), type II and type II ID) and other three genotypes and I / D alleles in peripheral blood of patients with CRC frequency, and with 120 cases of normal control test results were compared. Results: The distribution of ACE gene insertions / deletions (I / D) in peripheral blood samples of CRC patients was 15.00%, 50.00% and 35.00%, respectively. The frequencies of I / D allele frequencies were 60.00% and 40.00%, respectively. The distribution frequency of control group was 16.67%, 53.33% and 30.00%, respectively. The I / D allele frequencies were 56.67% and 43.33% There was no significant difference in the distribution frequency of the three genotypes (P> 0.05), but the frequency of I / D allele distribution was not significantly different (P> 0.05). Hierarchical analysis showed that D allele variation was associated with poorly differentiated CRC and distant metastasis (P <0.01 and P <0.05). Conclusion: The ACE gene I / D polymorphism has a certain relationship with CRC. The abnormality of D allele may be associated with the poorly differentiated and metastatic CRC.