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目的观察豆根管食通口服液对食管癌模型大鼠细胞凋亡及免疫逃逸的影响,从分子生物学角度分析其作用机制。方法健康Wistar大鼠随机选择15只为空白组,其余大鼠均腹部皮下注射甲基戊基亚硝胺5mg/kg建立食管癌模型大鼠,将造模成功的75只大鼠随机分为模型组、高剂量组、中剂量组、低剂量组、替加氟组各15只,低、中、高剂量组分别按5g/kg、10g/kg、15g/kg给予豆根管食通口服液,空白组、模型组给等予容积生理盐水,替加氟组给予替加氟片20mg/kg,各组均于造模成功24h后灌胃给药,每日1次,连续4周。末次给药24h后取瘤检测,原位末端标记法检测细胞凋亡,免疫组织化学法检测Fas、FasL蛋白表达。结果高、中、低剂量组及替加氟组食管癌细胞的凋亡指数与空白组、模型组比较差异均有统计学意义(P<0.05),并且高剂量组明显优于替加氟组、低剂量组(P<0.05)。模型组Fas蛋白表达阳性率低于空白组、FasL蛋白表达高于空白组(P<0.05或P<0.01);各治疗组Fas蛋白表达阳性率均高于模型组(P<0.05),高剂量组Fas蛋白表达阳性率高于替加氟组和低剂量组(P<0.05);高、中、低剂量组FasL蛋白表达阳性率明显低于模型组(P<0.05或P<0.01)。结论豆根管食通口服液能促进模型大鼠食管癌细胞凋亡,其作用机制可能与其提高Fas表达、降低FasL表达有关,并且存在一定的量效关系,高剂量的豆根管食通口服液作用优于替加氟片。
Objective To observe the effect of Doulu Guanxitong Oral Liquid on apoptosis and immune escape in esophageal cancer model rats and to analyze its mechanism from the perspective of molecular biology. Methods Fifteen healthy Wistar rats were randomly selected as the blank group. The remaining rats were injected subcutaneously with methyl pentylnitrosamine 5 mg / kg to establish esophageal cancer model rats, and 75 successful rats were randomly divided into model Group, high dose group, medium dose group, low dose group and tegafur group. The low, medium and high dose groups were administered with 5g / kg, 10g / kg and 15g / The rats in the blank group and the model group were given equal volume of normal saline. The tegafur group was given Tegafur tablet 20mg / kg, and the rats in each group were given gavage 24h after the model was established successfully, once a day for 4 weeks. Twenty-four hours after the last administration, tumor detection was performed. Apoptotic cells were detected by in situ end labeling. Fas, FasL protein expressions were detected by immunohistochemistry. Results The apoptotic index of esophageal cancer cells in high, medium and low dose groups and tegafur group were significantly different from those in blank group and model group (P <0.05), and were significantly higher in high dose group than in tegafur group , Low dose group (P <0.05). The positive rate of Fas protein in model group was lower than that in blank group, while the expression of FasL protein was higher in blank group than in blank group (P <0.05 or P <0.01). The positive rate of Fas protein in each treatment group was higher than that in model group (P <0.05) The positive rate of Fas protein expression was higher in the high, medium and low dose groups than that in the tegafur group and the low dose group (P <0.05 or P <0.01). Conclusion Douwatangsutong oral liquid can promote esophageal cancer cell apoptosis in model rats, and its mechanism may be related to increasing Fas expression and decreasing FasL expression, and there is a certain dose-effect relationship. Better than Teflon tablets.