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采用ELISA、IFA检测特异性抗体,MCPENT检测中和抗体和HI,进行血清学分型。结果:选择姬鼠型疫区,接种沙鼠肾细胞Ⅰ型灭活出血热疫苗安全性较好。免疫后42天HFRS.V.IgG抗体阳性率为9643%,GMT=3518,180天有明显下降,加强免疫两周后阳性率达100%,而且1200天仍有3636%人群存在1∶10(+)抗体;MCPENT抗体较IFA抗体阳性率低,但疫苗免疫人群具有特异性回忆反应。中期流行病学防病效果接种组发病率为235/10万,对照组为4293/10万,保护率9453%。
Specific antibodies were detected by ELISA, IFA, neutralizing antibodies by MCPENT and HI, serological typing. Results: It is safe to choose type Ⅱ inactivated murine hemorrhagic fever gerbils to infect the gerbil kidney cells. 42 days after immunization HFRS. V. IgG antibody positive rate of 96 43%, GMT = 35 18,180 days decreased significantly, two weeks after boosting the positive rate of 100%, and 1200 days still 36 36% of the population there is 1:10 (+), Antibody; MCPENT antibody IFA antibody-positive rate is low, but the vaccination of the crowd has a specific memory response. Epidemiological epidemiological prevention effect inoculation group incidence of 2 35/10 million, the control group was 42 93/10 million, the protection rate of 94 53%.