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本研究目的是想测定宫颈粘液过氧化物酶(CMP)的活性,与月经周期各激素变化的定量关系,以监视妇女排卵时间。作者选择40例年龄19～29岁,周期为26～32天的健康妇女,每人连续观察3个月,试验前禁用化学避孕药至少6个月。试验期间禁止性生活,以排除干扰因素。在每个月经周期的第5,9～15,17～19天收集粘液称重。同时取静脉血10～20ml,分离血清,两样均放置-20C°待用。取宫颈粘液5～500mg,用带有 Teflon 栓的玻璃匀浆器匀化,粘液溶液在加入0.5ml 二甲基-亚砜的0.5ml20%Ficoll 液内匀化;另一试验用 Fic-0ll/tritonx-100溶解粘液,液化的 CMP 置-20℃
The purpose of this study was to determine the quantitative relationship between cervical mucinous peroxidase (CMP) activity and various hormonal changes during the menstrual cycle to monitor women’s ovulation time. The authors selected 40 healthy women aged 19-29 years with a period of 26-32 days, each of whom was observed continuously for 3 months. The chemical contraceptives were banned for at least 6 months before the test. Sexual life is forbidden during the trial to exclude interference. Mucus was collected on the 5th, 9th, 15th, 17th to 19th day of each menstrual cycle. At the same time take venous blood 10 ~ 20ml, serum separation, both were placed -20C ° stand-by. Cervical mucus 5 ~ 500mg, homogenized with a glass homogenizer with a Teflon plug, the mucus solution homogenized in 0.5ml of 20% Ficoll solution containing 0.5ml of dimethyl sulfoxide; another test with Fic-0ll / Tritonx-100 dissolved mucus, liquefied CMP set -20 ℃