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研究了沼液与生防菌配比发酵液滴施对棉花产量及根际土壤微生物(细菌、真菌和放线菌)数量及酶(过氧化氢酶、脲酶、蔗糖酶和蛋白酶)活性的影响。土壤中真菌、细菌、放线菌分别采用马丁氏培养基、NA培养基和高氏一号培养基来测量;过氧化氢酶采用高锰酸钾容量法,脲酶采用NH4+释放量法,蔗糖酶采用3.5-二硝基水杨酸法,蛋白酶采用茚三酮比色法。结果表明:随着滴灌次数的增加,不同处理的棉花根际土壤中微生物数量和酶活性均呈先升后降的变化趋势,沼液生防菌发酵液、沼液和生防菌单独发酵液处理后棉花产量比对照分别增加44.16%、5.62%和7.42%。沼液生防菌发酵液比生防拮抗菌单一施用能最大程度改善土壤质量,并提高了棉花根际微生物数量和土壤酶的活性,增加棉花产量。
The effects of biogas slurry and biocontrol agent on the cotton yield and rhizosphere soil microorganisms (bacteria, fungi and actinomycetes) and the activity of enzymes (catalase, urease, invertase and protease) were studied . The soil fungi, bacteria, actinomycetes were measured using Martin’s medium, NA medium and Gao’s first medium; catalase using potassium permanganate capacity method, urease NH4 + release amount method, sucrase Using 3.5-dinitrosalicylic acid method, protease ninhydrin colorimetry. The results showed that with the increase of drip irrigation number, the microbial quantity and enzyme activity in rhizosphere soils of different treatments showed the trend of first increasing and then decreasing. The biocontrol bacterial fermentation broth, biogas slurry and biocontrol broth After treatment, the yield of cotton increased by 44.16%, 5.62% and 7.42% respectively compared with the control. Biogas biocontrol fermentation broth than the biocontrol antagonistic bacteria single application can maximize soil quality and improve cotton rhizosphere microbial activity and soil enzyme activity and increase cotton yield.