论文部分内容阅读
The prediction of the peak width at half height is an important aspect in the optimization ofthe chromatographic operating conditions.In this paper,a linear relationship,between the peakwidths at half height and the retention values with various isocratic elution is observed.In gradientelution,however,the relationship between the peak widths at half height and the so-called inventedretention values that correspond to the mobile phase composition by eluting the solute from the columnend is developed.We believe that there is almost the same band width at half height inside the column(in unit of length)for different solutes.The peak width at half height in the chromatogram(in unit oftime)is mainly determined by the capacity factor of the solute when it is eluted front the columnend.The larger the capacity factor of a solute eluted from the column end,the more slowly will bethe solute eluted from the column end and the wider will be the peak width at half height.It ispossible to predict the peak width at half height in various isocratic and gradient elutions by usingthis linear relationship.
The prediction of the peak width at half height is an important aspect in the optimization of the chromatographic operating conditions. In this paper, a linear relationship, between the peak widths at half height and the retention values with various isocratic elution is observed. Gradientelution, however , the relationship between the peak widths at half height and the so-called inventedretention values that correspond to the mobile phase composition by eluting the solute from the columnend is developed. We believe that there is almost the same band width at half height inside the column (in unit of length) for different solutes. The peak width at half height in the chromatogram (in unit of time) is mainly determined by the capacity factor of the solute when it is eluted front the columnend. the larger capacity factor of a solute eluted from the column end, the more slowly will be the solute eluted from the column end and the wider will be the peak width at half height. It is sensible to predict th e peak width at half height in various isocratic and gradient elutions by usingthis linear relationship.