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本实验采用含1/10,000戊二醛的0.05M,pH9.6碳酸盐缓冲液稀释抗体包被反应板,并使酶标记抗体浓度由1/100提高到1/50,使实验时间由常规方法的20多小时缩短到6小时。1∶320,000稀释的标定的人新鲜血痕浸液仍能得出阳性结果。本实验除用聚苯乙烯反应板外,还使用了聚氯乙烯反应板。两种反应板的实验条件及结果相同。聚氯乙烯反应板可以根据实验所需反应孔的多少进行剪裁,从而使实验材料得到节约。这两种反应板都可以预先用抗体包被后,置-20℃冰箱保存备用,从而简化了操作。本方法快速、灵敏、简单、重复性好,便于推广。
This experiment using 1 / 10,000 glutaraldehyde 0.05M, pH9.6 carbonate buffer diluted antibody coated reaction plate, and the enzyme labeled antibody concentration from 1/100 to 1/50, the experimental time from the conventional Method of more than 20 hours shortened to 6 hours. The 1: 320,000 dilution of the labeled human fresh bloodstream still yielded positive results. In addition to the experiment with polystyrene reaction plate, but also the use of PVC plate. The experimental conditions and the results of the two reaction plates are the same. PVC plate can be based on the number of reaction wells required for tailoring, so that experimental materials are saved. Both plates can be pre-coated with antibody and stored at -20 ° C for later use, simplifying operation. The method is fast, sensitive, simple, good repeatability, easy to promote.