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目的寻求一种适宜的石棉表面改性剂.方法利用流式细胞技术测定细胞周期以及NORTHERN杂交技术测定C?FOS癌基因转录活性,观察几种化合物对温石棉促人胚肺(HEL)细胞增殖作用的影响.结果将HEL细胞与温石棉共同孵育后,其S期细胞所占比例增加,C?FOS癌基因的转录活性也明显增强.用3种化合物预处理的石棉组,其S期细胞所占比例以及C?FOS癌基因的转录活性均低于未处理石棉组.结论用上述3种化合物预处理石棉,均可不同程度地抑制石棉的促细胞增殖作用.“,”Objective To seek for an ideal chemical for surface modification of asbestos.Methods Cell cycles were measured with flow cytometry and activity of c fos oncogene transcription was determined by Northern blotting technique to observe the effects of several kinds of chemicals on cell proliferation cycles in human embryo lung (HEL) cells induced by chrysotile.Results Proportion of HEL cells in the S phase,as well as activity of transcription of...