Phenotypic and Genetic Characterization ofBacillusSpecies Exhibiting Strong Proteolytic Activity Iso

来源 :东北农业大学学报(英文版) | 被引量 : 0次 | 上传用户:luhaixiong1971
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In this study, twoBacillistrains namely S2-3 and S4-5, isolated fromTerasi, a traditional fermented seafood product of Indonesia, were studied in terms of their phenotypic and genotypic properties. Both strains are of great interests due to their high proteolytic activity. Initially, they were subjected to morphological determination and a series of biochemical tests. These bacteria were gram-positive, endospore-formingBacilli. Based on 16S rRNA gene sequence analysis, the identities of the strains S2-3 and S4-5 were confirmed asBacillus thuringiensisandB. subtilis, respectively. Additionally, the two strains were also evaluated for their antibiogram profiles. It was found that they were susceptible to chloramphenicol, erythromycin, kanamycin, tetracycline and vancomycin and resistant to ampicillin and intermediately susceptible to bacitracin.
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